A conformational checkpoint between DNA binding and cleavage by CRISPR-Cas9

The Cas9 endonuclease is widely used for genome engineering applications by programming its single-guide RNA, and ongoing work is aimed at improving the accuracy and efficiency of DNA targeting. DNA cleavage of Cas9 is controlled by the conformational state of the HNH nuclease domain, but the mechan...

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Detalles Bibliográficos
Autores principales: Dagdas, Yavuz S., Chen, Janice S., Sternberg, Samuel H., Doudna, Jennifer A., Yildiz, Ahmet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2017
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547770/
https://www.ncbi.nlm.nih.gov/pubmed/28808686
http://dx.doi.org/10.1126/sciadv.aao0027

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547770/
https://www.ncbi.nlm.nih.gov/pubmed/28808686
http://dx.doi.org/10.1126/sciadv.aao0027

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