Engineered Cpf1 variants with altered PAM specificities increase genome targeting range

The RNA-guided endonuclease Cpf1 is a promising tool for genome editing in eukaryotic cells(1–7). However, the utility of the commonly used Acidaminococcus sp. BV3L6 Cpf1 (AsCpf1) and Lachnospiraceae bacterium ND2006 Cpf1 (LbCpf1) is limited by their requirement of a TTTV protospacer adjacent motif...

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Detalles Bibliográficos
Autores principales: Gao, Linyi, Cox, David B.T., Yan, Winston X., Manteiga, John C., Schneider, Martin W., Yamano, Takashi, Nishimasu, Hiroshi, Nureki, Osamu, Crosetto, Nicola, Zhang, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5548640/
https://www.ncbi.nlm.nih.gov/pubmed/28581492
http://dx.doi.org/10.1038/nbt.3900

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5548640/
https://www.ncbi.nlm.nih.gov/pubmed/28581492
http://dx.doi.org/10.1038/nbt.3900

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