DNA Heat Treatment for Improving qPCR Analysis of Human Adenovirus in Wastewater

PCR inhibitory substances in complex sample matrices can cause false negatives or under-estimation of target concentration. This study assessed DNA heat treatment for reducing inhibition during qPCR analysis of human adenovirus (HAdV) in wastewater samples. Inhibition was reduced by heat treating DN...

Descripción completa

Detalles Bibliográficos
Autores principales: Rames, Emily, Roiko, Anne, Stratton, Helen, Macdonald, Joanne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2017
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5548852/
https://www.ncbi.nlm.nih.gov/pubmed/28396977
http://dx.doi.org/10.1007/s12560-017-9294-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5548852/
https://www.ncbi.nlm.nih.gov/pubmed/28396977
http://dx.doi.org/10.1007/s12560-017-9294-4

Ejemplares similares