Chimeric proteins tagged with specific 3xHA cassettes may present instability and functional problems

Epitope-tagging of proteins has become a widespread technique for the analysis of protein function, protein interactions and protein localization among others. Tagging of genes by chromosomal integration of PCR amplified cassettes is a widely used and fast method to label proteins in vivo. Different...

Descripción completa

Detalles Bibliográficos
Autores principales: Saiz-Baggetto, Sara, Méndez, Ester, Quilis, Inma, Igual, J. Carlos, Bañó, M. Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5553802/
https://www.ncbi.nlm.nih.gov/pubmed/28800621
http://dx.doi.org/10.1371/journal.pone.0183067

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5553802/
https://www.ncbi.nlm.nih.gov/pubmed/28800621
http://dx.doi.org/10.1371/journal.pone.0183067

Ejemplares similares