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Chimeric proteins tagged with specific 3xHA cassettes may present instability and functional problems
Epitope-tagging of proteins has become a widespread technique for the analysis of protein function, protein interactions and protein localization among others. Tagging of genes by chromosomal integration of PCR amplified cassettes is a widely used and fast method to label proteins in vivo. Different...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5553802/ https://www.ncbi.nlm.nih.gov/pubmed/28800621 http://dx.doi.org/10.1371/journal.pone.0183067 |