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Recombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette
Gene manipulation is essential for metabolic engineering and synthetic biology, but the current general gene manipulation methods are not applicable to the non-model strain Corynebacterium glutamicum (C. glutamicum) ATCC14067, which is used for amino acid production. Here, we report an effective and...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5554157/ https://www.ncbi.nlm.nih.gov/pubmed/28801604 http://dx.doi.org/10.1038/s41598-017-08352-9 |