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The tomato histone deacetylase SlHDA1 contributes to the repression of fruit ripening and carotenoid accumulation

Histone deacetylation is one of the well characterized post-translational modifications related to transcriptional repression in eukaryotes. The process of histone deacetylation is achieved by histone deacetylases (HDACs). Over the last decade, substantial advances in our understanding of the mechan...

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Autores principales: Guo, Jun-E, Hu, Zongli, Zhu, Mingku, Li, Fenfen, Zhu, Zhiguo, Lu, Yu, Chen, Guoping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5554242/
https://www.ncbi.nlm.nih.gov/pubmed/28801625
http://dx.doi.org/10.1038/s41598-017-08512-x
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author Guo, Jun-E
Hu, Zongli
Zhu, Mingku
Li, Fenfen
Zhu, Zhiguo
Lu, Yu
Chen, Guoping
author_facet Guo, Jun-E
Hu, Zongli
Zhu, Mingku
Li, Fenfen
Zhu, Zhiguo
Lu, Yu
Chen, Guoping
author_sort Guo, Jun-E
collection PubMed
description Histone deacetylation is one of the well characterized post-translational modifications related to transcriptional repression in eukaryotes. The process of histone deacetylation is achieved by histone deacetylases (HDACs). Over the last decade, substantial advances in our understanding of the mechanism of fruit ripening have been achieved, but the role of HDACs in this process has not been elucidated. In our study, an RNA interference (RNAi) expression vector targeting SlHDA1 was constructed and transformed into tomato plants. Shorter fruit ripening time and decreased storability were observed in SlHDA1 RNAi lines. The accumulation of carotenoid was increased through an alteration of the carotenoid pathway flux. Ethylene content, ethylene biosynthesis genes (ACS2, ACS4 and ACO1, ACO3) and ripening-associated genes (RIN, E4, E8, Cnr, TAGL1, PG, Pti4 and LOXB) were significantly up-regulated in SlHDA1 RNAi lines. In addition, the expression of fruit cell wall metabolism genes (HEX, MAN, TBG4, XTH5 and XYL) was enhanced compared with wild type. Furthermore, SlHDA1 RNAi seedlings displayed shorter hypocotyls and were more sensitive to ACC (1-aminocyclopropane-1-carboxylate) than the wild type. The results of our study indicate that SlHDA1 functions as a negative regulator of fruit ripening by affecting ethylene synthesis and carotenoid accumulation.
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spelling pubmed-55542422017-08-15 The tomato histone deacetylase SlHDA1 contributes to the repression of fruit ripening and carotenoid accumulation Guo, Jun-E Hu, Zongli Zhu, Mingku Li, Fenfen Zhu, Zhiguo Lu, Yu Chen, Guoping Sci Rep Article Histone deacetylation is one of the well characterized post-translational modifications related to transcriptional repression in eukaryotes. The process of histone deacetylation is achieved by histone deacetylases (HDACs). Over the last decade, substantial advances in our understanding of the mechanism of fruit ripening have been achieved, but the role of HDACs in this process has not been elucidated. In our study, an RNA interference (RNAi) expression vector targeting SlHDA1 was constructed and transformed into tomato plants. Shorter fruit ripening time and decreased storability were observed in SlHDA1 RNAi lines. The accumulation of carotenoid was increased through an alteration of the carotenoid pathway flux. Ethylene content, ethylene biosynthesis genes (ACS2, ACS4 and ACO1, ACO3) and ripening-associated genes (RIN, E4, E8, Cnr, TAGL1, PG, Pti4 and LOXB) were significantly up-regulated in SlHDA1 RNAi lines. In addition, the expression of fruit cell wall metabolism genes (HEX, MAN, TBG4, XTH5 and XYL) was enhanced compared with wild type. Furthermore, SlHDA1 RNAi seedlings displayed shorter hypocotyls and were more sensitive to ACC (1-aminocyclopropane-1-carboxylate) than the wild type. The results of our study indicate that SlHDA1 functions as a negative regulator of fruit ripening by affecting ethylene synthesis and carotenoid accumulation. Nature Publishing Group UK 2017-08-11 /pmc/articles/PMC5554242/ /pubmed/28801625 http://dx.doi.org/10.1038/s41598-017-08512-x Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Guo, Jun-E
Hu, Zongli
Zhu, Mingku
Li, Fenfen
Zhu, Zhiguo
Lu, Yu
Chen, Guoping
The tomato histone deacetylase SlHDA1 contributes to the repression of fruit ripening and carotenoid accumulation
title The tomato histone deacetylase SlHDA1 contributes to the repression of fruit ripening and carotenoid accumulation
title_full The tomato histone deacetylase SlHDA1 contributes to the repression of fruit ripening and carotenoid accumulation
title_fullStr The tomato histone deacetylase SlHDA1 contributes to the repression of fruit ripening and carotenoid accumulation
title_full_unstemmed The tomato histone deacetylase SlHDA1 contributes to the repression of fruit ripening and carotenoid accumulation
title_short The tomato histone deacetylase SlHDA1 contributes to the repression of fruit ripening and carotenoid accumulation
title_sort tomato histone deacetylase slhda1 contributes to the repression of fruit ripening and carotenoid accumulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5554242/
https://www.ncbi.nlm.nih.gov/pubmed/28801625
http://dx.doi.org/10.1038/s41598-017-08512-x
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