Assessing glycolytic flux alterations resulting from genetic perturbations in E. coli using a biosensor

We describe the development of an optimized glycolytic flux biosensor and its application in detecting altered flux in a production strain and in a mutant library. The glycolytic flux biosensor is based on the Cra-regulated ppsA promoter of E. coli controlling fluorescent protein synthesis. We valid...

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Detalles Bibliográficos
Autores principales: Lehning, Christina E., Siedler, Solvej, Ellabaan, Mostafa M.H., Sommer, Morten O.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5555440/
https://www.ncbi.nlm.nih.gov/pubmed/28709932
http://dx.doi.org/10.1016/j.ymben.2017.07.002

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5555440/
https://www.ncbi.nlm.nih.gov/pubmed/28709932
http://dx.doi.org/10.1016/j.ymben.2017.07.002

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