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Assessing glycolytic flux alterations resulting from genetic perturbations in E. coli using a biosensor
We describe the development of an optimized glycolytic flux biosensor and its application in detecting altered flux in a production strain and in a mutant library. The glycolytic flux biosensor is based on the Cra-regulated ppsA promoter of E. coli controlling fluorescent protein synthesis. We valid...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Academic Press
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5555440/ https://www.ncbi.nlm.nih.gov/pubmed/28709932 http://dx.doi.org/10.1016/j.ymben.2017.07.002 |