Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens

The adaptation of CRISPR/SpCas9 technology to mammalian cell lines is transforming the study of human functional genomics. Pooled libraries of CRISPR guide RNAs (gRNAs) targeting human protein-coding genes and encoded in viral vectors have been used to systematically create gene knockouts in a varie...

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Detalles Bibliográficos
Autores principales: Hart, Traver, Tong, Amy Hin Yan, Chan, Katie, Van Leeuwen, Jolanda, Seetharaman, Ashwin, Aregger, Michael, Chandrashekhar, Megha, Hustedt, Nicole, Seth, Sahil, Noonan, Avery, Habsid, Andrea, Sizova, Olga, Nedyalkova, Lyudmila, Climie, Ryan, Tworzyanski, Leanne, Lawson, Keith, Sartori, Maria Augusta, Alibeh, Sabriyeh, Tieu, David, Masud, Sanna, Mero, Patricia, Weiss, Alexander, Brown, Kevin R., Usaj, Matej, Billmann, Maximilian, Rahman, Mahfuzur, Costanzo, Michael, Myers, Chad L., Andrews, Brenda J., Boone, Charles, Durocher, Daniel, Moffat, Jason
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2017
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5555476/
https://www.ncbi.nlm.nih.gov/pubmed/28655737
http://dx.doi.org/10.1534/g3.117.041277

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5555476/
https://www.ncbi.nlm.nih.gov/pubmed/28655737
http://dx.doi.org/10.1534/g3.117.041277

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