Molecular characterization of two novel echovirus 18 recombinants associated with hand-foot-mouth disease

Human echovirus 18 (E-18) is a member of the enterovirus B species. To date, sixteen full-length genome sequences of E-18 are available in the GenBank database. In this study, we describe the complete genomic characterization of two E-18 strains isolated in Yunnan, China. Pairwise comparisons of the...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Haihao, Zhao, Yilin, Liu, Hongbo, Sun, Hao, Huang, Xiaoqin, Yang, Zhaoqing, Ma, Shaohui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5559515/
https://www.ncbi.nlm.nih.gov/pubmed/28814774
http://dx.doi.org/10.1038/s41598-017-09038-y
_version_ 1783257532378120192
author Zhang, Haihao
Zhao, Yilin
Liu, Hongbo
Sun, Hao
Huang, Xiaoqin
Yang, Zhaoqing
Ma, Shaohui
author_facet Zhang, Haihao
Zhao, Yilin
Liu, Hongbo
Sun, Hao
Huang, Xiaoqin
Yang, Zhaoqing
Ma, Shaohui
author_sort Zhang, Haihao
collection PubMed
description Human echovirus 18 (E-18) is a member of the enterovirus B species. To date, sixteen full-length genome sequences of E-18 are available in the GenBank database. In this study, we describe the complete genomic characterization of two E-18 strains isolated in Yunnan, China. Pairwise comparisons of the nucleotide sequences and the deduced amino acid sequences revealed that the two Yunnan E-18 strains had 87.5% nucleotide identity and 96.3–96.5% amino acid identity with the Chinese strain. Phylogenetic and bootscanning analyses revealed the two E-18 strains had the highest identity with other several EV-B serotypes than the other E-18 strains in the P3 coding region, especially, 3B region of the Swine Vesicular disease virus (SVDV) strain HK70, indicated that frequent intertypic recombination might have occurred in the two Yunnan strains. This study contributes the complete genome sequences of E-18 to the GenBank database and provides valuable information on the molecular epidemiology of E-18 in China.
format Online
Article
Text
id pubmed-5559515
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-55595152017-08-18 Molecular characterization of two novel echovirus 18 recombinants associated with hand-foot-mouth disease Zhang, Haihao Zhao, Yilin Liu, Hongbo Sun, Hao Huang, Xiaoqin Yang, Zhaoqing Ma, Shaohui Sci Rep Article Human echovirus 18 (E-18) is a member of the enterovirus B species. To date, sixteen full-length genome sequences of E-18 are available in the GenBank database. In this study, we describe the complete genomic characterization of two E-18 strains isolated in Yunnan, China. Pairwise comparisons of the nucleotide sequences and the deduced amino acid sequences revealed that the two Yunnan E-18 strains had 87.5% nucleotide identity and 96.3–96.5% amino acid identity with the Chinese strain. Phylogenetic and bootscanning analyses revealed the two E-18 strains had the highest identity with other several EV-B serotypes than the other E-18 strains in the P3 coding region, especially, 3B region of the Swine Vesicular disease virus (SVDV) strain HK70, indicated that frequent intertypic recombination might have occurred in the two Yunnan strains. This study contributes the complete genome sequences of E-18 to the GenBank database and provides valuable information on the molecular epidemiology of E-18 in China. Nature Publishing Group UK 2017-08-16 /pmc/articles/PMC5559515/ /pubmed/28814774 http://dx.doi.org/10.1038/s41598-017-09038-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhang, Haihao
Zhao, Yilin
Liu, Hongbo
Sun, Hao
Huang, Xiaoqin
Yang, Zhaoqing
Ma, Shaohui
Molecular characterization of two novel echovirus 18 recombinants associated with hand-foot-mouth disease
title Molecular characterization of two novel echovirus 18 recombinants associated with hand-foot-mouth disease
title_full Molecular characterization of two novel echovirus 18 recombinants associated with hand-foot-mouth disease
title_fullStr Molecular characterization of two novel echovirus 18 recombinants associated with hand-foot-mouth disease
title_full_unstemmed Molecular characterization of two novel echovirus 18 recombinants associated with hand-foot-mouth disease
title_short Molecular characterization of two novel echovirus 18 recombinants associated with hand-foot-mouth disease
title_sort molecular characterization of two novel echovirus 18 recombinants associated with hand-foot-mouth disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5559515/
https://www.ncbi.nlm.nih.gov/pubmed/28814774
http://dx.doi.org/10.1038/s41598-017-09038-y
work_keys_str_mv AT zhanghaihao molecularcharacterizationoftwonovelechovirus18recombinantsassociatedwithhandfootmouthdisease
AT zhaoyilin molecularcharacterizationoftwonovelechovirus18recombinantsassociatedwithhandfootmouthdisease
AT liuhongbo molecularcharacterizationoftwonovelechovirus18recombinantsassociatedwithhandfootmouthdisease
AT sunhao molecularcharacterizationoftwonovelechovirus18recombinantsassociatedwithhandfootmouthdisease
AT huangxiaoqin molecularcharacterizationoftwonovelechovirus18recombinantsassociatedwithhandfootmouthdisease
AT yangzhaoqing molecularcharacterizationoftwonovelechovirus18recombinantsassociatedwithhandfootmouthdisease
AT mashaohui molecularcharacterizationoftwonovelechovirus18recombinantsassociatedwithhandfootmouthdisease

Ejemplares similares