Mechanism of transformation in Mycobacteria using a novel shockwave assisted technique driven by in-situ generated oxyhydrogen

We present a novel method for shockwave-assisted bacterial transformation using a miniature oxyhydrogen detonation-driven shock tube. We have obtained transformation efficiencies of about 1.28 × 10(6), 1.7 × 10(6), 5 × 10(6), 1 × 10(5), 1 × 10(5) and 2 × 10(5) transformants/µg of DNA for Escherichia...

Descripción completa

Detalles Bibliográficos
Autores principales: Datey, Akshay, Subburaj, Janardhanraj, Gopalan, Jagadeesh, Chakravortty, Dipshikha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5561244/
https://www.ncbi.nlm.nih.gov/pubmed/28819202
http://dx.doi.org/10.1038/s41598-017-08542-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5561244/
https://www.ncbi.nlm.nih.gov/pubmed/28819202
http://dx.doi.org/10.1038/s41598-017-08542-5

Ejemplares similares