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Amine Landscaping to Maximize Protein-Dye Fluorescence and Ultrastable Protein-Ligand Interaction
Chemical modification of proteins provides great opportunities to control and visualize living systems. The most common way to modify proteins is reaction of their abundant amines with N-hydroxysuccinimide (NHS) esters. Here we explore the impact of amine number and positioning on protein-conjugate...
Autores principales: | Jacobsen, Michael T., Fairhead, Michael, Fogelstrand, Per, Howarth, Mark |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5563079/ https://www.ncbi.nlm.nih.gov/pubmed/28757182 http://dx.doi.org/10.1016/j.chembiol.2017.06.015 |
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