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CrY2H-seq: a massively-multiplexed assay for deep coverage interactome mapping
Broad-scale protein-protein interaction mapping is a major challenge given the cost, time, and sensitivity constraints of existing technologies. Here, we present a massively-multiplexed yeast two-hybrid method, CrY2H-seq, that uses a Cre recombinase interaction reporter to intracellularly fuse the c...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564216/ https://www.ncbi.nlm.nih.gov/pubmed/28650476 http://dx.doi.org/10.1038/nmeth.4343 |