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Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

Cells constantly change their membrane architecture and protein distribution, but it is extremely difficult to visualize these events at a temporal and spatial resolution on the order of ms and nm, respectively. We have developed a time-resolved electron microscopy technique, "flash-and-freeze,...

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Detalles Bibliográficos
Autores principales: Li, Shuo, Raychaudhuri, Sumana, Watanabe, Shigeki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5565139/
https://www.ncbi.nlm.nih.gov/pubmed/28518090
http://dx.doi.org/10.3791/55664