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Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling

Microvascular endothelial cell heterogeneity and its relationship to hemodynamics remains poorly understood due to a lack of sufficient methods to examine these parameters in vivo at high resolution throughout an angiogenic network. The availability of surrogate markers for functional vascular prote...

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Autores principales: Kelly-Goss, Molly R., Ning, Bo, Bruce, Anthony C., Tavakol, Daniel N., Yi, David, Hu, Song, Yates, Paul A., Peirce, Shayn M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5567377/
https://www.ncbi.nlm.nih.gov/pubmed/28831080
http://dx.doi.org/10.1038/s41598-017-08982-z
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author Kelly-Goss, Molly R.
Ning, Bo
Bruce, Anthony C.
Tavakol, Daniel N.
Yi, David
Hu, Song
Yates, Paul A.
Peirce, Shayn M.
author_facet Kelly-Goss, Molly R.
Ning, Bo
Bruce, Anthony C.
Tavakol, Daniel N.
Yi, David
Hu, Song
Yates, Paul A.
Peirce, Shayn M.
author_sort Kelly-Goss, Molly R.
collection PubMed
description Microvascular endothelial cell heterogeneity and its relationship to hemodynamics remains poorly understood due to a lack of sufficient methods to examine these parameters in vivo at high resolution throughout an angiogenic network. The availability of surrogate markers for functional vascular proteins, such as green fluorescent protein, enables expression in individual cells to be followed over time using confocal microscopy, while photoacoustic microscopy enables dynamic measurement of blood flow across the network with capillary-level resolution. We combined these two non-invasive imaging modalities in order to spatially and temporally analyze biochemical and biomechanical drivers of angiogenesis in murine corneal neovessels. By stimulating corneal angiogenesis with an alkali burn in Tie2-GFP fluorescent-reporter mice, we evaluated how onset of blood flow and surgically-altered blood flow affects Tie2-GFP expression. Our study establishes a novel platform for analyzing heterogeneous blood flow and fluorescent reporter protein expression across a dynamic microvascular network in an adult mammal.
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spelling pubmed-55673772017-09-01 Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling Kelly-Goss, Molly R. Ning, Bo Bruce, Anthony C. Tavakol, Daniel N. Yi, David Hu, Song Yates, Paul A. Peirce, Shayn M. Sci Rep Article Microvascular endothelial cell heterogeneity and its relationship to hemodynamics remains poorly understood due to a lack of sufficient methods to examine these parameters in vivo at high resolution throughout an angiogenic network. The availability of surrogate markers for functional vascular proteins, such as green fluorescent protein, enables expression in individual cells to be followed over time using confocal microscopy, while photoacoustic microscopy enables dynamic measurement of blood flow across the network with capillary-level resolution. We combined these two non-invasive imaging modalities in order to spatially and temporally analyze biochemical and biomechanical drivers of angiogenesis in murine corneal neovessels. By stimulating corneal angiogenesis with an alkali burn in Tie2-GFP fluorescent-reporter mice, we evaluated how onset of blood flow and surgically-altered blood flow affects Tie2-GFP expression. Our study establishes a novel platform for analyzing heterogeneous blood flow and fluorescent reporter protein expression across a dynamic microvascular network in an adult mammal. Nature Publishing Group UK 2017-08-22 /pmc/articles/PMC5567377/ /pubmed/28831080 http://dx.doi.org/10.1038/s41598-017-08982-z Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kelly-Goss, Molly R.
Ning, Bo
Bruce, Anthony C.
Tavakol, Daniel N.
Yi, David
Hu, Song
Yates, Paul A.
Peirce, Shayn M.
Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling
title Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling
title_full Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling
title_fullStr Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling
title_full_unstemmed Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling
title_short Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling
title_sort dynamic, heterogeneous endothelial tie2 expression and capillary blood flow during microvascular remodeling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5567377/
https://www.ncbi.nlm.nih.gov/pubmed/28831080
http://dx.doi.org/10.1038/s41598-017-08982-z
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