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A two-stage temperature control strategy enhances extracellular secretion of recombinant α-cyclodextrin glucosyltransferase in Escherichia coli

The effects of temperature on extracellular secretion of the α-cyclodextrin glucosyltransferase (α-CGTase) from Paenibacillus macerans JFB05-01 by Escherichia coli were investigated. When protein expression was induced at constant temperature, the greatest amount of extracellular recombinant α-CGTas...

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Detalles Bibliográficos
Autores principales: Li, Yang, Liu, Jia, Wang, Yinglan, Liu, Bingjie, Xie, Xiaofang, Jia, Rui, Li, Caiming, Li, Zhaofeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5567581/
https://www.ncbi.nlm.nih.gov/pubmed/28831769
http://dx.doi.org/10.1186/s13568-017-0465-3