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A two-stage temperature control strategy enhances extracellular secretion of recombinant α-cyclodextrin glucosyltransferase in Escherichia coli
The effects of temperature on extracellular secretion of the α-cyclodextrin glucosyltransferase (α-CGTase) from Paenibacillus macerans JFB05-01 by Escherichia coli were investigated. When protein expression was induced at constant temperature, the greatest amount of extracellular recombinant α-CGTas...
Autores principales: | Li, Yang, Liu, Jia, Wang, Yinglan, Liu, Bingjie, Xie, Xiaofang, Jia, Rui, Li, Caiming, Li, Zhaofeng |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5567581/ https://www.ncbi.nlm.nih.gov/pubmed/28831769 http://dx.doi.org/10.1186/s13568-017-0465-3 |
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