Phenotyping first-generation genome editing mutants: a new standard?

The unprecedented efficiency of the CRISPR/Cas9 system in genome engineering has opened the prospect of employing mutant founders for phenotyping cohorts, thus accelerating research projects by circumventing the requirement to generate cohorts using conventional two- or three-generation crosses. How...

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Detalles Bibliográficos
Autores principales: Teboul, Lydia, Murray, Stephen A., Nolan, Patrick M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5569115/
https://www.ncbi.nlm.nih.gov/pubmed/28756587
http://dx.doi.org/10.1007/s00335-017-9711-x
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author Teboul, Lydia
Murray, Stephen A.
Nolan, Patrick M.
author_facet Teboul, Lydia
Murray, Stephen A.
Nolan, Patrick M.
author_sort Teboul, Lydia
collection PubMed
description The unprecedented efficiency of the CRISPR/Cas9 system in genome engineering has opened the prospect of employing mutant founders for phenotyping cohorts, thus accelerating research projects by circumventing the requirement to generate cohorts using conventional two- or three-generation crosses. However, these first-generation mutants are often genetic mosaics, with a complex and difficult to define genetic make-up. Here, we discuss the potential benefits, challenges and scientific validity of such models.
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spelling pubmed-55691152017-09-07 Phenotyping first-generation genome editing mutants: a new standard? Teboul, Lydia Murray, Stephen A. Nolan, Patrick M. Mamm Genome Article The unprecedented efficiency of the CRISPR/Cas9 system in genome engineering has opened the prospect of employing mutant founders for phenotyping cohorts, thus accelerating research projects by circumventing the requirement to generate cohorts using conventional two- or three-generation crosses. However, these first-generation mutants are often genetic mosaics, with a complex and difficult to define genetic make-up. Here, we discuss the potential benefits, challenges and scientific validity of such models. Springer US 2017-07-29 2017 /pmc/articles/PMC5569115/ /pubmed/28756587 http://dx.doi.org/10.1007/s00335-017-9711-x Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Teboul, Lydia
Murray, Stephen A.
Nolan, Patrick M.
Phenotyping first-generation genome editing mutants: a new standard?
title Phenotyping first-generation genome editing mutants: a new standard?
title_full Phenotyping first-generation genome editing mutants: a new standard?
title_fullStr Phenotyping first-generation genome editing mutants: a new standard?
title_full_unstemmed Phenotyping first-generation genome editing mutants: a new standard?
title_short Phenotyping first-generation genome editing mutants: a new standard?
title_sort phenotyping first-generation genome editing mutants: a new standard?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5569115/
https://www.ncbi.nlm.nih.gov/pubmed/28756587
http://dx.doi.org/10.1007/s00335-017-9711-x
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