An optimised protocol for isolation of RNA from small sections of laser-capture microdissected FFPE tissue amenable for next-generation sequencing

BACKGROUND: Formalin-fixed paraffin embedded (FFPE) tissue constitutes a vast treasury of samples for biomedical research. Thus far however, extraction of RNA from FFPE tissue has proved challenging due to chemical RNA–protein crosslinking and RNA fragmentation, both of which heavily impact on RNA q...

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Detalles Bibliográficos
Autores principales: Amini, Parisa, Ettlin, Julia, Opitz, Lennart, Clementi, Elena, Malbon, Alexandra, Markkanen, Enni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5569520/
https://www.ncbi.nlm.nih.gov/pubmed/28835206
http://dx.doi.org/10.1186/s12867-017-0099-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5569520/
https://www.ncbi.nlm.nih.gov/pubmed/28835206
http://dx.doi.org/10.1186/s12867-017-0099-7

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