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In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR

The precise assembly of defined DNA sequences into plasmids is an essential task in bioscience research. While a number of molecular cloning techniques have been developed, many methods require specialized expensive reagents or laborious experimental procedure. Not surprisingly, conventional cloning...

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Detalles Bibliográficos
Autores principales: Huang, Faqing, Spangler, Joseph Rankin, Huang, Allen Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570364/
https://www.ncbi.nlm.nih.gov/pubmed/28837659
http://dx.doi.org/10.1371/journal.pone.0183974