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In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR
The precise assembly of defined DNA sequences into plasmids is an essential task in bioscience research. While a number of molecular cloning techniques have been developed, many methods require specialized expensive reagents or laborious experimental procedure. Not surprisingly, conventional cloning...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2017
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570364/ https://www.ncbi.nlm.nih.gov/pubmed/28837659 http://dx.doi.org/10.1371/journal.pone.0183974 |