Transcriptome Assembly and Systematic Identification of Novel Cytochrome P450s in Taxus chinensis

Taxus spp. is a highly valuable medicinal plant with multiple pharmacological effects on various cancers. Cytochrome P450s (CYP450s) play important roles in the biosynthesis of active compounds in Taxus spp., such as the famous diterpenoid, Taxol. However, some specific CYP450 enzymes involved in th...

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Autores principales: Liao, Weifang, Zhao, Shengying, Zhang, Meng, Dong, Kaige, Chen, Ying, Fu, Chunhua, Yu, Longjiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5572210/
https://www.ncbi.nlm.nih.gov/pubmed/28878800
http://dx.doi.org/10.3389/fpls.2017.01468
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author Liao, Weifang
Zhao, Shengying
Zhang, Meng
Dong, Kaige
Chen, Ying
Fu, Chunhua
Yu, Longjiang
author_facet Liao, Weifang
Zhao, Shengying
Zhang, Meng
Dong, Kaige
Chen, Ying
Fu, Chunhua
Yu, Longjiang
author_sort Liao, Weifang
collection PubMed
description Taxus spp. is a highly valuable medicinal plant with multiple pharmacological effects on various cancers. Cytochrome P450s (CYP450s) play important roles in the biosynthesis of active compounds in Taxus spp., such as the famous diterpenoid, Taxol. However, some specific CYP450 enzymes involved in the biosynthesis of Taxol remain unknown, and the systematic identification of CYP450s in Taxus has not been reported. In this study, 118 full-length and 175 partial CYP450 genes were identified in Taxus chinensis transcriptomes. The 118 full-length genes were divided into 8 clans and 29 families. The CYP71 clan included all A-type genes (52) belonging to 11 families. The other seven clans possessed 18 families containing 66 non-A-type genes. Two new gymnosperm-specific families were discovered, and were named CYP864 and CYP947 respectively. Protein sequence alignments revealed that all of the T. chinensis CYP450s hold distinct conserved domains. The expression patterns of all 118 CYP450 genes during the long-time subculture and MeJA elicitation were analyzed. Additionally, the expression levels of 15 novel CYP725 genes in different Taxus species were explored. Considering all the evidence, 6 CYP725s were identified to be candidates for Taxol biosynthesis. The cis-regulatory elements involved in the transcriptional regulation were also identified in the promoter regions of CYP725s. This study presents a comprehensive overview of the CYP450 gene family in T. chinensis and can provide important insights into the functional gene studies of Taxol biosynthesis.
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spelling pubmed-55722102017-09-06 Transcriptome Assembly and Systematic Identification of Novel Cytochrome P450s in Taxus chinensis Liao, Weifang Zhao, Shengying Zhang, Meng Dong, Kaige Chen, Ying Fu, Chunhua Yu, Longjiang Front Plant Sci Plant Science Taxus spp. is a highly valuable medicinal plant with multiple pharmacological effects on various cancers. Cytochrome P450s (CYP450s) play important roles in the biosynthesis of active compounds in Taxus spp., such as the famous diterpenoid, Taxol. However, some specific CYP450 enzymes involved in the biosynthesis of Taxol remain unknown, and the systematic identification of CYP450s in Taxus has not been reported. In this study, 118 full-length and 175 partial CYP450 genes were identified in Taxus chinensis transcriptomes. The 118 full-length genes were divided into 8 clans and 29 families. The CYP71 clan included all A-type genes (52) belonging to 11 families. The other seven clans possessed 18 families containing 66 non-A-type genes. Two new gymnosperm-specific families were discovered, and were named CYP864 and CYP947 respectively. Protein sequence alignments revealed that all of the T. chinensis CYP450s hold distinct conserved domains. The expression patterns of all 118 CYP450 genes during the long-time subculture and MeJA elicitation were analyzed. Additionally, the expression levels of 15 novel CYP725 genes in different Taxus species were explored. Considering all the evidence, 6 CYP725s were identified to be candidates for Taxol biosynthesis. The cis-regulatory elements involved in the transcriptional regulation were also identified in the promoter regions of CYP725s. This study presents a comprehensive overview of the CYP450 gene family in T. chinensis and can provide important insights into the functional gene studies of Taxol biosynthesis. Frontiers Media S.A. 2017-08-23 /pmc/articles/PMC5572210/ /pubmed/28878800 http://dx.doi.org/10.3389/fpls.2017.01468 Text en Copyright © 2017 Liao, Zhao, Zhang, Dong, Chen, Fu and Yu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Liao, Weifang
Zhao, Shengying
Zhang, Meng
Dong, Kaige
Chen, Ying
Fu, Chunhua
Yu, Longjiang
Transcriptome Assembly and Systematic Identification of Novel Cytochrome P450s in Taxus chinensis
title Transcriptome Assembly and Systematic Identification of Novel Cytochrome P450s in Taxus chinensis
title_full Transcriptome Assembly and Systematic Identification of Novel Cytochrome P450s in Taxus chinensis
title_fullStr Transcriptome Assembly and Systematic Identification of Novel Cytochrome P450s in Taxus chinensis
title_full_unstemmed Transcriptome Assembly and Systematic Identification of Novel Cytochrome P450s in Taxus chinensis
title_short Transcriptome Assembly and Systematic Identification of Novel Cytochrome P450s in Taxus chinensis
title_sort transcriptome assembly and systematic identification of novel cytochrome p450s in taxus chinensis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5572210/
https://www.ncbi.nlm.nih.gov/pubmed/28878800
http://dx.doi.org/10.3389/fpls.2017.01468
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