Evolutionary conserved role of eukaryotic translation factor eIF5A in the regulation of actin-nucleating formins

Elongation factor eIF5A is required for the translation of consecutive prolines, and was shown in yeast to translate polyproline-containing Bni1, an actin-nucleating formin required for polarized growth during mating. Here we show that Drosophila eIF5A can functionally replace yeast eIF5A and is req...

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Autores principales: Muñoz-Soriano, Verónica, Domingo-Muelas, Ana, Li, Tianlu, Gamero, Esther, Bizy, Alexandra, Fariñas, Isabel, Alepuz, Paula, Paricio, Nuria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575014/
https://www.ncbi.nlm.nih.gov/pubmed/28852021
http://dx.doi.org/10.1038/s41598-017-10057-y
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author Muñoz-Soriano, Verónica
Domingo-Muelas, Ana
Li, Tianlu
Gamero, Esther
Bizy, Alexandra
Fariñas, Isabel
Alepuz, Paula
Paricio, Nuria
author_facet Muñoz-Soriano, Verónica
Domingo-Muelas, Ana
Li, Tianlu
Gamero, Esther
Bizy, Alexandra
Fariñas, Isabel
Alepuz, Paula
Paricio, Nuria
author_sort Muñoz-Soriano, Verónica
collection PubMed
description Elongation factor eIF5A is required for the translation of consecutive prolines, and was shown in yeast to translate polyproline-containing Bni1, an actin-nucleating formin required for polarized growth during mating. Here we show that Drosophila eIF5A can functionally replace yeast eIF5A and is required for actin-rich cable assembly during embryonic dorsal closure (DC). Furthermore, Diaphanous, the formin involved in actin dynamics during DC, is regulated by and mediates eIF5A effects. Finally, eIF5A controls cell migration and regulates Diaphanous levels also in mammalian cells. Our results uncover an evolutionary conserved role of eIF5A regulating cytoskeleton-dependent processes through translation of formins in eukaryotes.
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spelling pubmed-55750142017-09-01 Evolutionary conserved role of eukaryotic translation factor eIF5A in the regulation of actin-nucleating formins Muñoz-Soriano, Verónica Domingo-Muelas, Ana Li, Tianlu Gamero, Esther Bizy, Alexandra Fariñas, Isabel Alepuz, Paula Paricio, Nuria Sci Rep Article Elongation factor eIF5A is required for the translation of consecutive prolines, and was shown in yeast to translate polyproline-containing Bni1, an actin-nucleating formin required for polarized growth during mating. Here we show that Drosophila eIF5A can functionally replace yeast eIF5A and is required for actin-rich cable assembly during embryonic dorsal closure (DC). Furthermore, Diaphanous, the formin involved in actin dynamics during DC, is regulated by and mediates eIF5A effects. Finally, eIF5A controls cell migration and regulates Diaphanous levels also in mammalian cells. Our results uncover an evolutionary conserved role of eIF5A regulating cytoskeleton-dependent processes through translation of formins in eukaryotes. Nature Publishing Group UK 2017-08-29 /pmc/articles/PMC5575014/ /pubmed/28852021 http://dx.doi.org/10.1038/s41598-017-10057-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Muñoz-Soriano, Verónica
Domingo-Muelas, Ana
Li, Tianlu
Gamero, Esther
Bizy, Alexandra
Fariñas, Isabel
Alepuz, Paula
Paricio, Nuria
Evolutionary conserved role of eukaryotic translation factor eIF5A in the regulation of actin-nucleating formins
title Evolutionary conserved role of eukaryotic translation factor eIF5A in the regulation of actin-nucleating formins
title_full Evolutionary conserved role of eukaryotic translation factor eIF5A in the regulation of actin-nucleating formins
title_fullStr Evolutionary conserved role of eukaryotic translation factor eIF5A in the regulation of actin-nucleating formins
title_full_unstemmed Evolutionary conserved role of eukaryotic translation factor eIF5A in the regulation of actin-nucleating formins
title_short Evolutionary conserved role of eukaryotic translation factor eIF5A in the regulation of actin-nucleating formins
title_sort evolutionary conserved role of eukaryotic translation factor eif5a in the regulation of actin-nucleating formins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575014/
https://www.ncbi.nlm.nih.gov/pubmed/28852021
http://dx.doi.org/10.1038/s41598-017-10057-y
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