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Site-specific incorporation of phosphotyrosine using an expanded genetic code
Access to phosphoproteins with stoichiometric and site-specific phosphorylation status is key for understanding the role of protein phosphorylation. Here we report an efficient method to generate pure, active phosphotyrosine-containing proteins by genetically encoding a stable phosphotyrosine analog...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5577362/ https://www.ncbi.nlm.nih.gov/pubmed/28604697 http://dx.doi.org/10.1038/nchembio.2406 |