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Interaction between Various Apple Procyanidin and Staphylococcal Enterotoxin A and Their Inhibitory Effects on Toxin Activity

In this study, we investigated the interaction between apple polyphenols (AP; mainly consisting of procyanidin (PC) from an apple) and staphylococcal enterotoxin A (SEA), and the inhibitory effects of AP on SEA activity. According to the degree of polymerization, in particularly highly polymerized P...

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Autores principales: Shimamura, Yuko, Hirai, Chikako, Sugiyama, Yuka, Utsumi, Mio, Yanagida, Akio, Murata, Masatsune, Ohashi, Norio, Masuda, Shuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5577577/
https://www.ncbi.nlm.nih.gov/pubmed/28783092
http://dx.doi.org/10.3390/toxins9080243
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author Shimamura, Yuko
Hirai, Chikako
Sugiyama, Yuka
Utsumi, Mio
Yanagida, Akio
Murata, Masatsune
Ohashi, Norio
Masuda, Shuichi
author_facet Shimamura, Yuko
Hirai, Chikako
Sugiyama, Yuka
Utsumi, Mio
Yanagida, Akio
Murata, Masatsune
Ohashi, Norio
Masuda, Shuichi
author_sort Shimamura, Yuko
collection PubMed
description In this study, we investigated the interaction between apple polyphenols (AP; mainly consisting of procyanidin (PC) from an apple) and staphylococcal enterotoxin A (SEA), and the inhibitory effects of AP on SEA activity. According to the degree of polymerization, in particularly highly polymerized PC (more than pentamer) strongly interacted with SEA. The binding affinity of AP with SEA molecules was determined using Biacore analysis. AP reacted with SEA immobilized on a Biacore sensor chip. After treatment with pepsin and pancreatin, to examine the changes of binding affinity of AP in intragastric conditions, AP maintained interaction with SEA. We examined whether AP inhibits the proliferation and interferon-γ (IFN-γ) production induced by SEA in mouse spleen cells. AP strongly inactivated the proliferation and IFN-γ production induced by SEA. These results suggest that AP, which has a higher degree of polymerization, inactivates stronger biological activity of SEA through interaction with SEA. Our studies are the first to demonstrate the relationship between the degree of polymerization of AP and the inhibitory effects on SEA activities.
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spelling pubmed-55775772017-09-05 Interaction between Various Apple Procyanidin and Staphylococcal Enterotoxin A and Their Inhibitory Effects on Toxin Activity Shimamura, Yuko Hirai, Chikako Sugiyama, Yuka Utsumi, Mio Yanagida, Akio Murata, Masatsune Ohashi, Norio Masuda, Shuichi Toxins (Basel) Article In this study, we investigated the interaction between apple polyphenols (AP; mainly consisting of procyanidin (PC) from an apple) and staphylococcal enterotoxin A (SEA), and the inhibitory effects of AP on SEA activity. According to the degree of polymerization, in particularly highly polymerized PC (more than pentamer) strongly interacted with SEA. The binding affinity of AP with SEA molecules was determined using Biacore analysis. AP reacted with SEA immobilized on a Biacore sensor chip. After treatment with pepsin and pancreatin, to examine the changes of binding affinity of AP in intragastric conditions, AP maintained interaction with SEA. We examined whether AP inhibits the proliferation and interferon-γ (IFN-γ) production induced by SEA in mouse spleen cells. AP strongly inactivated the proliferation and IFN-γ production induced by SEA. These results suggest that AP, which has a higher degree of polymerization, inactivates stronger biological activity of SEA through interaction with SEA. Our studies are the first to demonstrate the relationship between the degree of polymerization of AP and the inhibitory effects on SEA activities. MDPI 2017-08-07 /pmc/articles/PMC5577577/ /pubmed/28783092 http://dx.doi.org/10.3390/toxins9080243 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shimamura, Yuko
Hirai, Chikako
Sugiyama, Yuka
Utsumi, Mio
Yanagida, Akio
Murata, Masatsune
Ohashi, Norio
Masuda, Shuichi
Interaction between Various Apple Procyanidin and Staphylococcal Enterotoxin A and Their Inhibitory Effects on Toxin Activity
title Interaction between Various Apple Procyanidin and Staphylococcal Enterotoxin A and Their Inhibitory Effects on Toxin Activity
title_full Interaction between Various Apple Procyanidin and Staphylococcal Enterotoxin A and Their Inhibitory Effects on Toxin Activity
title_fullStr Interaction between Various Apple Procyanidin and Staphylococcal Enterotoxin A and Their Inhibitory Effects on Toxin Activity
title_full_unstemmed Interaction between Various Apple Procyanidin and Staphylococcal Enterotoxin A and Their Inhibitory Effects on Toxin Activity
title_short Interaction between Various Apple Procyanidin and Staphylococcal Enterotoxin A and Their Inhibitory Effects on Toxin Activity
title_sort interaction between various apple procyanidin and staphylococcal enterotoxin a and their inhibitory effects on toxin activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5577577/
https://www.ncbi.nlm.nih.gov/pubmed/28783092
http://dx.doi.org/10.3390/toxins9080243
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