The Effect of Reduced Graphene Oxide-Coated Biphasic Calcium Phosphate Bone Graft Material on Osteogenesis

This study was conducted to evaluate the effect of biphasic calcium phosphate (BCP) coated with reduced graphene oxide (rGO) as bone graft materials on bone regeneration. The rGO-coated BCP bone graft material was fabricatied by mixing rGO and BCP at various concentrations. The surface charge of rGO...

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Autores principales: Kim, Jeong-Woo, Shin, Yong Cheol, Lee, Jin-Ju, Bae, Eun-Bin, Jeon, Young-Chan, Jeong, Chang-Mo, Yun, Mi-Jung, Lee, So-Hyoun, Han, Dong-Wook, Huh, Jung-Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578115/
https://www.ncbi.nlm.nih.gov/pubmed/28786931
http://dx.doi.org/10.3390/ijms18081725
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author Kim, Jeong-Woo
Shin, Yong Cheol
Lee, Jin-Ju
Bae, Eun-Bin
Jeon, Young-Chan
Jeong, Chang-Mo
Yun, Mi-Jung
Lee, So-Hyoun
Han, Dong-Wook
Huh, Jung-Bo
author_facet Kim, Jeong-Woo
Shin, Yong Cheol
Lee, Jin-Ju
Bae, Eun-Bin
Jeon, Young-Chan
Jeong, Chang-Mo
Yun, Mi-Jung
Lee, So-Hyoun
Han, Dong-Wook
Huh, Jung-Bo
author_sort Kim, Jeong-Woo
collection PubMed
description This study was conducted to evaluate the effect of biphasic calcium phosphate (BCP) coated with reduced graphene oxide (rGO) as bone graft materials on bone regeneration. The rGO-coated BCP bone graft material was fabricatied by mixing rGO and BCP at various concentrations. The surface charge of rGO-coated BCP was measured to be −14.43 mV, which formed a static electrostatic interaction. Cell viabilities were significantly diminished at higher concentrations of ≥100 μg/mL. The calvarial defects of 48 rats were implanted rGO-coated BCPs at a weight ratio of 2:1000 (rGO2), 4:1000 (rGO4), and 10:1000 (rGO10), repectively. BCP was used as a control group. The micro-CT and histological analysis were performed to evaluate new bone formation at 2 and 8 weeks after surgery. The results showed that the new bone volume (mm(3)) was significantly higher in the experimental groups than in the control group. Histological analysis showed that new bone areas (%) were significantly higher in the rGO2 and rGO10 than in the control, and significantly higher in rGO4 than in the rGO2 and rGO10. Conclusively, the rGO-coated BCP was found to be effective on osteogenesis and the concentration of the composite was an important factor.
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spelling pubmed-55781152017-09-05 The Effect of Reduced Graphene Oxide-Coated Biphasic Calcium Phosphate Bone Graft Material on Osteogenesis Kim, Jeong-Woo Shin, Yong Cheol Lee, Jin-Ju Bae, Eun-Bin Jeon, Young-Chan Jeong, Chang-Mo Yun, Mi-Jung Lee, So-Hyoun Han, Dong-Wook Huh, Jung-Bo Int J Mol Sci Article This study was conducted to evaluate the effect of biphasic calcium phosphate (BCP) coated with reduced graphene oxide (rGO) as bone graft materials on bone regeneration. The rGO-coated BCP bone graft material was fabricatied by mixing rGO and BCP at various concentrations. The surface charge of rGO-coated BCP was measured to be −14.43 mV, which formed a static electrostatic interaction. Cell viabilities were significantly diminished at higher concentrations of ≥100 μg/mL. The calvarial defects of 48 rats were implanted rGO-coated BCPs at a weight ratio of 2:1000 (rGO2), 4:1000 (rGO4), and 10:1000 (rGO10), repectively. BCP was used as a control group. The micro-CT and histological analysis were performed to evaluate new bone formation at 2 and 8 weeks after surgery. The results showed that the new bone volume (mm(3)) was significantly higher in the experimental groups than in the control group. Histological analysis showed that new bone areas (%) were significantly higher in the rGO2 and rGO10 than in the control, and significantly higher in rGO4 than in the rGO2 and rGO10. Conclusively, the rGO-coated BCP was found to be effective on osteogenesis and the concentration of the composite was an important factor. MDPI 2017-08-08 /pmc/articles/PMC5578115/ /pubmed/28786931 http://dx.doi.org/10.3390/ijms18081725 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Jeong-Woo
Shin, Yong Cheol
Lee, Jin-Ju
Bae, Eun-Bin
Jeon, Young-Chan
Jeong, Chang-Mo
Yun, Mi-Jung
Lee, So-Hyoun
Han, Dong-Wook
Huh, Jung-Bo
The Effect of Reduced Graphene Oxide-Coated Biphasic Calcium Phosphate Bone Graft Material on Osteogenesis
title The Effect of Reduced Graphene Oxide-Coated Biphasic Calcium Phosphate Bone Graft Material on Osteogenesis
title_full The Effect of Reduced Graphene Oxide-Coated Biphasic Calcium Phosphate Bone Graft Material on Osteogenesis
title_fullStr The Effect of Reduced Graphene Oxide-Coated Biphasic Calcium Phosphate Bone Graft Material on Osteogenesis
title_full_unstemmed The Effect of Reduced Graphene Oxide-Coated Biphasic Calcium Phosphate Bone Graft Material on Osteogenesis
title_short The Effect of Reduced Graphene Oxide-Coated Biphasic Calcium Phosphate Bone Graft Material on Osteogenesis
title_sort effect of reduced graphene oxide-coated biphasic calcium phosphate bone graft material on osteogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578115/
https://www.ncbi.nlm.nih.gov/pubmed/28786931
http://dx.doi.org/10.3390/ijms18081725
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