Construction of an easy-to-use CRISPR-Cas9 system by patching a newly designed EXIT circuit

BACKGROUND: Plasmid-borne genetic editing tools, including the widely used CRISPR-Cas9 system, have greatly facilitated bacterial programming to obtain novel functionalities. However, the lack of effective post-editing plasmid elimination methods impedes follow-up genetic manipulation or application...

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Detalles Bibliográficos
Autores principales: Tang, Qiang, Lou, Chunbo, Liu, Shuang-Jiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5582390/
https://www.ncbi.nlm.nih.gov/pubmed/28878819
http://dx.doi.org/10.1186/s13036-017-0072-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5582390/
https://www.ncbi.nlm.nih.gov/pubmed/28878819
http://dx.doi.org/10.1186/s13036-017-0072-5

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