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Construction of an easy-to-use CRISPR-Cas9 system by patching a newly designed EXIT circuit
BACKGROUND: Plasmid-borne genetic editing tools, including the widely used CRISPR-Cas9 system, have greatly facilitated bacterial programming to obtain novel functionalities. However, the lack of effective post-editing plasmid elimination methods impedes follow-up genetic manipulation or application...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2017
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5582390/ https://www.ncbi.nlm.nih.gov/pubmed/28878819 http://dx.doi.org/10.1186/s13036-017-0072-5 |