Fluorescent CRISPR Adaptation Reporter for rapid quantification of spacer acquisition

CRISPR-Cas systems are adaptive prokaryotic immune systems protecting against horizontally transferred DNA or RNA such as viruses and other mobile genetic elements. Memory of past invaders is stored as spacers in CRISPR loci in a process called adaptation. Here we developed a novel assay where space...

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Detalles Bibliográficos
Autores principales: Amlinger, Lina, Hoekzema, Mirthe, Wagner, E. Gerhart H., Koskiniemi, Sanna, Lundgren, Magnus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5583386/
https://www.ncbi.nlm.nih.gov/pubmed/28871175
http://dx.doi.org/10.1038/s41598-017-10876-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5583386/
https://www.ncbi.nlm.nih.gov/pubmed/28871175
http://dx.doi.org/10.1038/s41598-017-10876-z

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