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Selection of reference genes suitable for normalization of qPCR data under abiotic stresses in bioenergy crop Arundo donax L.

Suitable reference gene selection in qRT-PCR is a key pre-requisite to produce reliable data in gene expression analyses. In this study, novel primers for six commonly used reference genes (AC1, TLF, Act2, TUB α, EF-1α and GAPDH) plus two new candidates (pDUF221 and RPN6) were designed and comparati...

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Detalles Bibliográficos
Autores principales: Poli, Michele, Salvi, Silvio, Li, Mingai, Varotto, Claudio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587670/
https://www.ncbi.nlm.nih.gov/pubmed/28878356
http://dx.doi.org/10.1038/s41598-017-11019-0