Halotolerant aminopeptidase M29 from Mesorhizobium SEMIA 3007 with biotechnological potential and its impact on biofilm synthesis

The aminopeptidase gene from Mesorhizobium SEMIA3007 was cloned and overexpressed in Escherichia coli. The enzyme called MesoAmp exhibited optimum activity at pH 8.5 and 45 °C and was strongly activated by Co(2+) and Mn(2+). Under these reaction conditions, the enzyme displayed K(m) and k(cat) value...

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Detalles Bibliográficos
Autores principales: Sierra, Elwi Machado, Pereira, Mariana Rangel, Maester, Thaís Carvalho, Gomes-Pepe, Elisangela Soares, Mendoza, Elkin Rodas, Lemos, Eliana G. de Macedo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587760/
https://www.ncbi.nlm.nih.gov/pubmed/28878230
http://dx.doi.org/10.1038/s41598-017-10932-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587760/
https://www.ncbi.nlm.nih.gov/pubmed/28878230
http://dx.doi.org/10.1038/s41598-017-10932-8

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