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Halotolerant aminopeptidase M29 from Mesorhizobium SEMIA 3007 with biotechnological potential and its impact on biofilm synthesis
The aminopeptidase gene from Mesorhizobium SEMIA3007 was cloned and overexpressed in Escherichia coli. The enzyme called MesoAmp exhibited optimum activity at pH 8.5 and 45 °C and was strongly activated by Co(2+) and Mn(2+). Under these reaction conditions, the enzyme displayed K(m) and k(cat) value...
Autores principales: | Sierra, Elwi Machado, Pereira, Mariana Rangel, Maester, Thaís Carvalho, Gomes-Pepe, Elisangela Soares, Mendoza, Elkin Rodas, Lemos, Eliana G. de Macedo |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587760/ https://www.ncbi.nlm.nih.gov/pubmed/28878230 http://dx.doi.org/10.1038/s41598-017-10932-8 |
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