mRNA detection in budding yeast with single fluorophores

Quantitative measurement of mRNA levels in single cells is necessary to understand phenotypic variability within an otherwise isogenic population of cells. Single-molecule mRNA Fluorescence In Situ Hybridization (FISH) has been established as the standard method for this purpose, but current protoco...

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Detalles Bibliográficos
Autores principales: Wadsworth, Gable M., Parikh, Rasesh Y., Choy, John S., Kim, Harold D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587780/
https://www.ncbi.nlm.nih.gov/pubmed/28666354
http://dx.doi.org/10.1093/nar/gkx568

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587780/
https://www.ncbi.nlm.nih.gov/pubmed/28666354
http://dx.doi.org/10.1093/nar/gkx568

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