Highly efficient preparation of single-stranded DNA rings by T4 ligase at abnormally low Mg(II) concentration

Preparation of large amount of single-stranded circular DNA in high selectivity is crucial for further developments of nanotechnology and other DNA sciences. Herein, a simple but practically useful methodology to prepare DNA rings has been presented. One of the essential factors is to use highly dil...

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Detalles Bibliográficos
Autores principales: An, Ran, Li, Qi, Fan, Yiqiao, Li, Jing, Pan, Xiaoming, Komiyama, Makoto, Liang, Xingguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587803/
https://www.ncbi.nlm.nih.gov/pubmed/28655200
http://dx.doi.org/10.1093/nar/gkx553

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587803/
https://www.ncbi.nlm.nih.gov/pubmed/28655200
http://dx.doi.org/10.1093/nar/gkx553

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