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Highly efficient preparation of single-stranded DNA rings by T4 ligase at abnormally low Mg(II) concentration

Preparation of large amount of single-stranded circular DNA in high selectivity is crucial for further developments of nanotechnology and other DNA sciences. Herein, a simple but practically useful methodology to prepare DNA rings has been presented. One of the essential factors is to use highly dil...

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Detalles Bibliográficos
Autores principales:	An, Ran, Li, Qi, Fan, Yiqiao, Li, Jing, Pan, Xiaoming, Komiyama, Makoto, Liang, Xingguo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2017
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587803/ https://www.ncbi.nlm.nih.gov/pubmed/28655200 http://dx.doi.org/10.1093/nar/gkx553

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