Engineering human PrimPol into an efficient RNA-dependent-DNA primase/polymerase

We have developed a straightforward fluorometric assay to measure primase-polymerase activity of human PrimPol (HsPrimPol). The sensitivity of this procedure uncovered a novel RNA-dependent DNA priming-polymerization activity (RdDP) of this enzyme. In an attempt to enhance HsPrimPol RdDP activity, w...

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Detalles Bibliográficos
Autores principales: Agudo, Rubén, Calvo, Patricia A., Martínez-Jiménez, María I., Blanco, Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587808/
https://www.ncbi.nlm.nih.gov/pubmed/28911121
http://dx.doi.org/10.1093/nar/gkx633

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587808/
https://www.ncbi.nlm.nih.gov/pubmed/28911121
http://dx.doi.org/10.1093/nar/gkx633

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