Cargando…

Synthesis and incorporation of (13)C-labeled DNA building blocks to probe structural dynamics of DNA by NMR

We report the synthesis of atom-specifically (13)C-modified building blocks that can be incorporated into DNA via solid phase synthesis to facilitate investigations on structural and dynamic features via NMR spectroscopy. In detail, 6-(13)C-modified pyrimidine and 8-(13)C purine DNA phosphoramidites...

Descripción completa

Detalles Bibliográficos
Autores principales:	Nußbaumer, Felix, Juen, Michael Andreas, Gasser, Catherina, Kremser, Johannes, Müller, Thomas, Tollinger, Martin, Kreutz, Christoph
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2017
Materias:	Structural Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587810/ https://www.ncbi.nlm.nih.gov/pubmed/28911104 http://dx.doi.org/10.1093/nar/gkx592

Ejemplares similares

Branch site bulge conformations in domain 6 determine functional sugar puckers in group II intron splicing
por: Plangger, Raphael, et al.
Publicado: (2019)

Excited States of Nucleic Acids Probed by Proton Relaxation Dispersion NMR Spectroscopy
por: Juen, Michael Andreas, et al.
Publicado: (2016)

Probing RNA dynamics via longitudinal exchange and CPMG relaxation dispersion NMR spectroscopy using a sensitive (13)C-methyl label
por: Kloiber, Karin, et al.
Publicado: (2011)

Aromatic (19)F–(13)C TROSY—[(19)F, (13)C]‐Pyrimidine Labeling for NMR Spectroscopy of RNA
por: Nußbaumer, Felix, et al.
Publicado: (2020)

Probing the mechanism of recognition of ssDNA by the Cdc13-DBD
por: Eldridge, Aimee M., et al.
Publicado: (2008)

The intrinsic mechanics of B-DNA in solution characterized by NMR
por: Imeddourene, Akli Ben, et al.
Publicado: (2016)

Probing Rad51-DNA interactions by changing DNA twist
por: Atwell, Scott, et al.
Publicado: (2012)

Probing the elastic limit of DNA bending
por: Le, Tung T., et al.
Publicado: (2014)

The structure of the SAM/SAH-binding riboswitch
por: Weickhmann, A Katharina, et al.
Publicado: (2019)

Measurement of Ligand–Target Residence Times by (1)H Relaxation Dispersion NMR Spectroscopy
por: Moschen, Thomas, et al.
Publicado: (2016)

5-Fluoro pyrimidines: labels to probe DNA and RNA secondary structures by 1D (19)F NMR spectroscopy
por: Puffer, Barbara, et al.
Publicado: (2009)

Snapshots of an evolved DNA polymerase pre- and post-incorporation of an unnatural nucleotide
por: Singh, Isha, et al.
Publicado: (2018)

Structural accommodation of ribonucleotide incorporation by the DNA repair enzyme polymerase Mu
por: Moon, Andrea F., et al.
Publicado: (2017)

NMR structural analysis of DNA recognition by a novel Myb1 DNA-binding domain in the protozoan parasite Trichomonas vaginalis
por: Lou, Yuan-Chao, et al.
Publicado: (2009)

Impact of 5-formylcytosine on the melting kinetics of DNA by (1)H NMR chemical exchange
por: Dubini, Romeo C A, et al.
Publicado: (2020)

NMR-based method of small changes reveals how DNA mutator APOBEC3A interacts with its single-stranded DNA substrate
por: Harjes, Stefan, et al.
Publicado: (2017)

NMR solution structure of tricyclo-DNA containing duplexes: insight into enhanced thermal stability and nuclease resistance
por: Istrate, Andrei, et al.
Publicado: (2019)

Unexpected A-form formation of 4′-thioDNA in solution, revealed by NMR, and the implications as to the mechanism of nuclease resistance
por: Matsugami, Akimasa, et al.
Publicado: (2008)

Solution structures of 2 : 1 and 1 : 1 DNA polymerase–DNA complexes probed by ultracentrifugation and small-angle X-ray scattering
por: Tang, Kuo-Hsiang, et al.
Publicado: (2008)

Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies
por: Olenginski, Lukasz T., et al.
Publicado: (2021)

5‐Oxyacetic Acid Modification Destabilizes Double Helical Stem Structures and Favors Anionic Watson–Crick like cmo(5)U‐G Base Pairs
por: Strebitzer, Elisabeth, et al.
Publicado: (2018)

NMR structure of a vestigial nuclease provides insight into the evolution of functional transitions in viral dsDNA packaging motors
por: Mahler, Bryon P, et al.
Publicado: (2020)

NMR solution structure of an asymmetric intermolecular leaped V-shape G-quadruplex: selective recognition of the d(G2NG3NG4) sequence motif by a short linear G-rich DNA probe
por: Wan, Chanjuan, et al.
Publicado: (2019)

Structure of an RNA aptamer in complex with the fluorophore tetramethylrhodamine
por: Duchardt-Ferner, Elke, et al.
Publicado: (2020)

Mismatched dNTP incorporation by DNA polymerase β does not proceed via globally different conformational pathways(†)
por: Tang, Kuo-Hsiang, et al.
Publicado: (2008)

A DNA minor groove electronegative potential genome map based on photo-chemical probing
por: Lindemose, Søren, et al.
Publicado: (2011)

Double threading through DNA: NMR structural study of a bis-naphthalene macrocycle bound to a thymine–thymine mismatch
por: Jourdan, Muriel, et al.
Publicado: (2012)

Structural and dynamic studies of DNA recognition by NF-κB p50 RHR homodimer using methyl NMR spectroscopy
por: Singh, Amrinder, et al.
Publicado: (2022)

Crystal structure of a DNA containing the planar, phenoxazine-derived bi-functional spectroscopic probe Ç
por: Edwards, Thomas E., et al.
Publicado: (2011)

Mechanical unfolding of human telomere G-quadruplex DNA probed by integrated fluorescence and magnetic tweezers spectroscopy
por: Long, Xi, et al.
Publicado: (2013)

Structural changes of an abasic site in duplex DNA affect noncovalent binding of the spin label ç
por: Shelke, Sandip A., et al.
Publicado: (2012)

High-resolution EPR distance measurements on RNA and DNA with the non-covalent Ǵ spin label
por: Heinz, Marcel, et al.
Publicado: (2020)

Thioctic Acid Derivatives as Building Blocks to Incorporate DNA Oligonucleotides onto Gold Nanoparticles
por: Pérez-Rentero, Sónia, et al.
Publicado: (2014)

Nucleotide binding interactions modulate dNTP selectivity and facilitate 8-oxo-dGTP incorporation by DNA polymerase lambda
por: Burak, Matthew J., et al.
Publicado: (2015)

Structural probing of the HIV-1 polypurine tract RNA:DNA hybrid using classic nucleic acid ligands
por: Turner, Kevin B., et al.
Publicado: (2008)

NMR solution and X-ray crystal structures of a DNA molecule containing both right- and left-handed parallel-stranded G-quadruplexes
por: Winnerdy, Fernaldo Richtia, et al.
Publicado: (2019)

Probing the Hydrogen-Bonding Environment of Individual Bases in DNA Duplexes with Isotope-Edited Infrared Spectroscopy
por: Fick, Robert J., et al.
Publicado: (2021)

Locked nucleic acid building blocks as versatile tools for advanced G-quadruplex design
por: Haase, Linn, et al.
Publicado: (2020)

Epigenetic CpG duplex marks probed by an evolved DNA reader via a well-tempered conformational plasticity
por: Singh, Himanshu, et al.
Publicado: (2023)

Phosphoramidite building blocks with protected nitroxides for the synthesis of spin-labeled DNA and RNA
por: Weinrich, Timo, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_3m3acc52tl7fdirvsbic1t91dd