Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans

Xylose is a general component of O-glycans in mammals. Core-xylosylation of N-glycans is only found in plants and helminth. Consequently, xylosylated N-glycans cause immunological response in humans. We have used the F-protein of the human respiratory syncytial virus (RSV), one of the main causes of...

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Autores principales: Sandig, Grit, von Horsten, Hans Henning, Radke, Lars, Blanchard, Véronique, Frohme, Marcus, Giese, Christoph, Sandig, Volker, Hinderlich, Stephan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5590453/
https://www.ncbi.nlm.nih.gov/pubmed/28952517
http://dx.doi.org/10.3390/bioengineering4020038
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author Sandig, Grit
von Horsten, Hans Henning
Radke, Lars
Blanchard, Véronique
Frohme, Marcus
Giese, Christoph
Sandig, Volker
Hinderlich, Stephan
author_facet Sandig, Grit
von Horsten, Hans Henning
Radke, Lars
Blanchard, Véronique
Frohme, Marcus
Giese, Christoph
Sandig, Volker
Hinderlich, Stephan
author_sort Sandig, Grit
collection PubMed
description Xylose is a general component of O-glycans in mammals. Core-xylosylation of N-glycans is only found in plants and helminth. Consequently, xylosylated N-glycans cause immunological response in humans. We have used the F-protein of the human respiratory syncytial virus (RSV), one of the main causes of respiratory tract infection in infants and elderly, as a model protein for vaccination. The RSV-F protein was expressed in CHO-DG44 cells, which were further modified by co-expression of β1,2-xylosyltransferase from Nicotiana tabacum. Xylosylation of RSV-F N-glycans was shown by monosaccharide analysis and MALDI-TOF mass spectrometry. In immunogenic studies with a human artificial lymph node model, the engineered RSV-F protein revealed improved vaccination efficacy.
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spelling pubmed-55904532017-09-21 Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans Sandig, Grit von Horsten, Hans Henning Radke, Lars Blanchard, Véronique Frohme, Marcus Giese, Christoph Sandig, Volker Hinderlich, Stephan Bioengineering (Basel) Article Xylose is a general component of O-glycans in mammals. Core-xylosylation of N-glycans is only found in plants and helminth. Consequently, xylosylated N-glycans cause immunological response in humans. We have used the F-protein of the human respiratory syncytial virus (RSV), one of the main causes of respiratory tract infection in infants and elderly, as a model protein for vaccination. The RSV-F protein was expressed in CHO-DG44 cells, which were further modified by co-expression of β1,2-xylosyltransferase from Nicotiana tabacum. Xylosylation of RSV-F N-glycans was shown by monosaccharide analysis and MALDI-TOF mass spectrometry. In immunogenic studies with a human artificial lymph node model, the engineered RSV-F protein revealed improved vaccination efficacy. MDPI 2017-04-28 /pmc/articles/PMC5590453/ /pubmed/28952517 http://dx.doi.org/10.3390/bioengineering4020038 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sandig, Grit
von Horsten, Hans Henning
Radke, Lars
Blanchard, Véronique
Frohme, Marcus
Giese, Christoph
Sandig, Volker
Hinderlich, Stephan
Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans
title Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans
title_full Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans
title_fullStr Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans
title_full_unstemmed Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans
title_short Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans
title_sort engineering of cho cells for the production of recombinant glycoprotein vaccines with xylosylated n-glycans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5590453/
https://www.ncbi.nlm.nih.gov/pubmed/28952517
http://dx.doi.org/10.3390/bioengineering4020038
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