Label-free quantitative (1)H NMR spectroscopy to study low-affinity ligand–protein interactions in solution: A contribution to the mechanism of polyphenol-mediated astringency

Nuclear magnetic resonance (NMR) spectroscopy is well-established in assessing the binding affinity between low molecular weight ligands and proteins. However, conventional NMR-based binding assays are often limited to small proteins of high purity and may require elaborate isotopic labeling of one...

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Detalles Bibliográficos
Autores principales: Delius, Judith, Frank, Oliver, Hofmann, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5590944/
https://www.ncbi.nlm.nih.gov/pubmed/28886151
http://dx.doi.org/10.1371/journal.pone.0184487

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5590944/
https://www.ncbi.nlm.nih.gov/pubmed/28886151
http://dx.doi.org/10.1371/journal.pone.0184487

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