HPLC-based quantification of bacterial housekeeping nucleotides and alarmone messengers ppGpp and pppGpp

Here we describe an HPLC-based method to quantify bacterial housekeeping nucleotides and the signaling messengers ppGpp and pppGpp. We have replicated and tested several previously reported HPLC-based approaches and assembled a method that can process 50 samples in three days, thus making kineticall...

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Detalles Bibliográficos
Autores principales: Varik, Vallo, Oliveira, Sofia Raquel Alves, Hauryliuk, Vasili, Tenson, Tanel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5591245/
https://www.ncbi.nlm.nih.gov/pubmed/28887466
http://dx.doi.org/10.1038/s41598-017-10988-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5591245/
https://www.ncbi.nlm.nih.gov/pubmed/28887466
http://dx.doi.org/10.1038/s41598-017-10988-6

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