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HPLC-based quantification of bacterial housekeeping nucleotides and alarmone messengers ppGpp and pppGpp
Here we describe an HPLC-based method to quantify bacterial housekeeping nucleotides and the signaling messengers ppGpp and pppGpp. We have replicated and tested several previously reported HPLC-based approaches and assembled a method that can process 50 samples in three days, thus making kineticall...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5591245/ https://www.ncbi.nlm.nih.gov/pubmed/28887466 http://dx.doi.org/10.1038/s41598-017-10988-6 |