Recycling of a selectable marker with a self-excisable plasmid in Pichia pastoris

Pichia pastoris is a widely used heterologous protein production workhorse. However, with its multiple genetic modifications to solve bottlenecks for heterologous protein productivity, P. pastoris lacks selectable markers. Existing selectable marker recycling plasmids have drawbacks (e.g., slow grow...

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Detalles Bibliográficos
Autores principales: Li, Cheng, Lin, Ying, Zheng, Xueyun, Yuan, Qingyan, Pang, Nuo, Liao, Xihao, Huang, Yuanyuan, Zhang, Xinying, Liang, Shuli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5593967/
https://www.ncbi.nlm.nih.gov/pubmed/28894268
http://dx.doi.org/10.1038/s41598-017-11494-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5593967/
https://www.ncbi.nlm.nih.gov/pubmed/28894268
http://dx.doi.org/10.1038/s41598-017-11494-5

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