Cas9 Nickase-Assisted RNA Repression Enables Stable and Efficient Manipulation of Essential Metabolic Genes in Clostridium cellulolyticum

Essential gene functions remain largely underexplored in bacteria. Clostridium cellulolyticum is a promising candidate for consolidated bioprocessing; however, its genetic manipulation to reduce the formation of less-valuable acetate is technically challenging due to the essentiality of acetate-prod...

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Detalles Bibliográficos
Autores principales: Xu, Tao, Li, Yongchao, He, Zhili, Van Nostrand, Joy D., Zhou, Jizhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5594222/
https://www.ncbi.nlm.nih.gov/pubmed/28936208
http://dx.doi.org/10.3389/fmicb.2017.01744

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5594222/
https://www.ncbi.nlm.nih.gov/pubmed/28936208
http://dx.doi.org/10.3389/fmicb.2017.01744

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