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Cas9 Nickase-Assisted RNA Repression Enables Stable and Efficient Manipulation of Essential Metabolic Genes in Clostridium cellulolyticum

Essential gene functions remain largely underexplored in bacteria. Clostridium cellulolyticum is a promising candidate for consolidated bioprocessing; however, its genetic manipulation to reduce the formation of less-valuable acetate is technically challenging due to the essentiality of acetate-prod...

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Detalles Bibliográficos
Autores principales:	Xu, Tao, Li, Yongchao, He, Zhili, Van Nostrand, Joy D., Zhou, Jizhong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2017
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5594222/ https://www.ncbi.nlm.nih.gov/pubmed/28936208 http://dx.doi.org/10.3389/fmicb.2017.01744

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