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Iron imaging reveals tumor and metastasis macrophage hemosiderin deposits in breast cancer

Iron-deposition is a metabolic biomarker of macrophages in both normal and pathological situations, but the presence of iron in tumor and metastasis-associated macrophages is not known. Here we mapped and quantified hemosiderin-laden macrophage (HLM) deposits in murine models of metastatic breast ca...

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Autores principales: Leftin, Avigdor, Ben-Chetrit, Nir, Klemm, Florian, Joyce, Johanna A., Koutcher, Jason A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5595304/
https://www.ncbi.nlm.nih.gov/pubmed/28898277
http://dx.doi.org/10.1371/journal.pone.0184765
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author Leftin, Avigdor
Ben-Chetrit, Nir
Klemm, Florian
Joyce, Johanna A.
Koutcher, Jason A.
author_facet Leftin, Avigdor
Ben-Chetrit, Nir
Klemm, Florian
Joyce, Johanna A.
Koutcher, Jason A.
author_sort Leftin, Avigdor
collection PubMed
description Iron-deposition is a metabolic biomarker of macrophages in both normal and pathological situations, but the presence of iron in tumor and metastasis-associated macrophages is not known. Here we mapped and quantified hemosiderin-laden macrophage (HLM) deposits in murine models of metastatic breast cancer using iron and macrophage histology, and in vivo MRI. Iron MRI detected high-iron pixel clusters in mammary tumors, lung metastasis, and brain metastasis as well as liver and spleen tissue known to contain the HLMs. Iron histology showed these regions to contain clustered macrophages identified by their common iron status and tissue-intrinsic association with other phenotypic macrophage markers. The in vivo MRI and ex vivo histological images were further processed to determine the frequencies and sizes of the iron deposits, and measure the number of HLMs in each deposit to estimate the in vivo MRI sensitivity for these cells. Hemosiderin accumulation is a macrophage biomarker and intrinsic contrast source for cellular MRI associated with the innate function of macrophages in iron metabolism systemically, and in metastatic cancer.
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spelling pubmed-55953042017-09-15 Iron imaging reveals tumor and metastasis macrophage hemosiderin deposits in breast cancer Leftin, Avigdor Ben-Chetrit, Nir Klemm, Florian Joyce, Johanna A. Koutcher, Jason A. PLoS One Research Article Iron-deposition is a metabolic biomarker of macrophages in both normal and pathological situations, but the presence of iron in tumor and metastasis-associated macrophages is not known. Here we mapped and quantified hemosiderin-laden macrophage (HLM) deposits in murine models of metastatic breast cancer using iron and macrophage histology, and in vivo MRI. Iron MRI detected high-iron pixel clusters in mammary tumors, lung metastasis, and brain metastasis as well as liver and spleen tissue known to contain the HLMs. Iron histology showed these regions to contain clustered macrophages identified by their common iron status and tissue-intrinsic association with other phenotypic macrophage markers. The in vivo MRI and ex vivo histological images were further processed to determine the frequencies and sizes of the iron deposits, and measure the number of HLMs in each deposit to estimate the in vivo MRI sensitivity for these cells. Hemosiderin accumulation is a macrophage biomarker and intrinsic contrast source for cellular MRI associated with the innate function of macrophages in iron metabolism systemically, and in metastatic cancer. Public Library of Science 2017-09-12 /pmc/articles/PMC5595304/ /pubmed/28898277 http://dx.doi.org/10.1371/journal.pone.0184765 Text en © 2017 Leftin et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Leftin, Avigdor
Ben-Chetrit, Nir
Klemm, Florian
Joyce, Johanna A.
Koutcher, Jason A.
Iron imaging reveals tumor and metastasis macrophage hemosiderin deposits in breast cancer
title Iron imaging reveals tumor and metastasis macrophage hemosiderin deposits in breast cancer
title_full Iron imaging reveals tumor and metastasis macrophage hemosiderin deposits in breast cancer
title_fullStr Iron imaging reveals tumor and metastasis macrophage hemosiderin deposits in breast cancer
title_full_unstemmed Iron imaging reveals tumor and metastasis macrophage hemosiderin deposits in breast cancer
title_short Iron imaging reveals tumor and metastasis macrophage hemosiderin deposits in breast cancer
title_sort iron imaging reveals tumor and metastasis macrophage hemosiderin deposits in breast cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5595304/
https://www.ncbi.nlm.nih.gov/pubmed/28898277
http://dx.doi.org/10.1371/journal.pone.0184765
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