Metabolic engineering for recombinant major ampullate spidroin 2 (MaSp2) synthesis in Escherichia coli

In this research, metabolic engineering was employed to synthesize the artificial major ampullate spidroin 2 (MaSp2) in the engineered Escherichia coli. An iterative seamless splicing strategy was used to assemble the MaSp2 gene, which could reach 10000 base pairs, and more than 100 kDa protein was...

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Detalles Bibliográficos
Autores principales: Cao, Hao, Parveen, Shafaq, Ding, Ding, Xu, Haijun, Tan, Tianwei, Liu, Luo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5595792/
https://www.ncbi.nlm.nih.gov/pubmed/28900203
http://dx.doi.org/10.1038/s41598-017-11845-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5595792/
https://www.ncbi.nlm.nih.gov/pubmed/28900203
http://dx.doi.org/10.1038/s41598-017-11845-2

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