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Metabolic engineering for recombinant major ampullate spidroin 2 (MaSp2) synthesis in Escherichia coli
In this research, metabolic engineering was employed to synthesize the artificial major ampullate spidroin 2 (MaSp2) in the engineered Escherichia coli. An iterative seamless splicing strategy was used to assemble the MaSp2 gene, which could reach 10000 base pairs, and more than 100 kDa protein was...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5595792/ https://www.ncbi.nlm.nih.gov/pubmed/28900203 http://dx.doi.org/10.1038/s41598-017-11845-2 |