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Limitations of Qdot labelling compared to directly-conjugated probes for single particle tracking of B cell receptor mobility

Single-particle tracking (SPT) is a powerful method for exploring single-molecule dynamics in living cells with nanoscale spatiotemporal resolution. Photostability and bright fluorescence make quantum dots (Qdots) a popular choice for SPT. However, their large size could potentially alter the mobili...

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Detalles Bibliográficos
Autores principales: Abraham, Libin, Lu, Henry Y., Falcão, Rebeca Cardim, Scurll, Joshua, Jou, Timothy, Irwin, Brian, Tafteh, Reza, Gold, Michael R., Coombs, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5595841/
https://www.ncbi.nlm.nih.gov/pubmed/28900238
http://dx.doi.org/10.1038/s41598-017-11563-9