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Limitations of Qdot labelling compared to directly-conjugated probes for single particle tracking of B cell receptor mobility
Single-particle tracking (SPT) is a powerful method for exploring single-molecule dynamics in living cells with nanoscale spatiotemporal resolution. Photostability and bright fluorescence make quantum dots (Qdots) a popular choice for SPT. However, their large size could potentially alter the mobili...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5595841/ https://www.ncbi.nlm.nih.gov/pubmed/28900238 http://dx.doi.org/10.1038/s41598-017-11563-9 |